PROF. Christine Foyer recognized as a Redox Pioneer

Dr. Christine Foyer (B.Sc. 1974; Ph.D. 1977) has been recognized as a Redox Pioneer because she has published an article on redox biology that has been cited more than 1000 times, 4 other articles that have been cited more than 500 times, and a further 32 articles that have been each cited more than 100 times. During her Ph.D. at the Kings College, University of London, United Kingdom, Dr. Foyer discovered that ascorbate and glutathione and enzymes linking NADPH, glutathione, and ascorbate are localized in isolated chloroplast preparations. These observations pioneered the discovery of the ascorbate-glutathione cycle, now known as Foyer-Halliwell-Asada pathway after the names of the three major contributors, a crucial mechanism for H2O2 metabolism in both animals and plants. Dr. Foyer has made a very significant contribution to our current understanding of the crucial roles of ascorbate and glutathione in redox biology, particularly in relation to photosynthesis, respiration, and chloroplast and mitochondrial redox signaling networks. Antioxid. Redox Signal. 15, 2383 – 2391.

My view is that science...is compulsive and you have to keep with it all the time and not get despondent when things do not work well. Being passionate about science is what carries you through the hard times so that it isn't so much work, as a hobby that you do for a living. It is the thrill of achieving a better understanding and finding real pleasure in putting new ideas together, explaining data and passing on knowledge that keeps you going no matter what!


– Prof. Christine Helen Foyer

Biotechnology and Entrepreneurship at University of Pretoria

An all-female team of young entrepreneurship students has won the first ever Biotechnology in the Workplace competition at the University of Pretoria on the weekend of November 12th – 14th, 2010. The competition was the culmination of an Honours course designed to teach students about entrepreneurship in the biological sciences. After an intensive weekend of seminars and mentoring sessions with South African and international entrepreneurship experts, four groups of students competed by pitching their business plans to an independent panel of " investors " .

The winning team of Degracious Kgoale (Research and Development Director), Lindiwe Nkosi (Marketing Director), Monique Heystek (Finance Director) and Magdeleen du Plessis (Managing Director), with Prof. Karl Kunert.

" Fruit Loot, " the winning hypothetical company, was an agricultural biotech firm targeting the fruit growing market of South Africa. Their key product " Branchstop " was a biologically produced strigolactone spray which could be applied to trees to stop excessive lateral branching, thereby increasing the fruit yield of orchard trees up to 80%. The company had a strong intellectual property portfolio and an impressive marketing strategy targeting their customer base through print ads and fruit growers' unions. The presented business pitch included a realistic financial plan, expansion routes and exit strategies which convinced the judging panel.

The winning team presented their convincing business plan with great enthusiasm. They showed good teamwork during the workshop and were a strong unit which handled the rigorous questions of the judges very well.
The winning group is pictured with Professor Karl Kunert (Univ Pretoria) who organised the honours module on Biotechnology in the Workplace in conjunction with Prof Jonathan Youngelson (Head of Innovation Support). The entrepreneurship weekend was supported by the British Council, in collaboration with the John Innes Centre, Norwich UK.
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Visit to Chonbuk National University in Jeonju, South Korea, March 2009

New Publications

Mbedzi PP, Van den Hooven L, Vorster BJ , Van der Waals J. (2019) Screening for Sclerotinia sclerotiorum resistance using detached leaf assays and simple sequence repeat markers in soybean cultivars. Crop Protection 215:104909. 10.1016/j.cropro.2019.104909 PDF
Kibido T, Kunert K, Makgopa M, Greve M, Vorster BJ . (2019) Improvement of rhizobium‐soybean symbiosis and nitrogen fixation under drought. Food and Energy Security :e117. 10.1002/fes3.177 PDF
Juan Vorster, Chris Cullis, Karl Kunert. (2019) Plant Vacuolar Processing Enzymes. Frontiers in Plant Science :00497. 10.3389/fpls.2019.00479 PDF
Cullis C, Lawler DW, Chimwamurombe P, Kunert K, Bbebe N, Vorster J. (2019) Development of marama bean, an orphan legume, as a crop. Food and Energy Security e00164:1-12. 10.1002/fes3.164 PDF
NM Okumu, BJ Vorster, CF Reinhardt. (2019) Growth-stage and temperature influence glyphosate resistance in Conyza bonariensis (L.) Cronquist.. South African Journal of Botany 121:248-256. 10.1016/j.sajb.2018.10.034 PDF
Cristopher Cullis, Percy Chimwamurombe, Nigel Barker, Karl Kunert, Juan Vorster. (2018) Orphan Legumes Growing in Dry Environments: Marama Bean as a Case Study. Frontiers in Plant Science 9:11999. 10.3389/fpls.2018.01199 PDF
Magdeleen Cilliers, Stefan van Wyk, Phillipus van Heerden, Karl Kunert, Juan Vorster. (2018) Identification and changes of the drought-induced cysteine protease transcriptome in soybean (Glycine max) root nodules. Journal of Experimental and Environmental Botany 148:59-69. 10.1016/j.envexpbot.2017.12.005
Priyen Pillay, Karl Kunert, Eugene Makgopa, Chris Cullis, Juan Vorste. (2016) Agroinfiltration contributes to VP1 recombinant protein degradation. Bioengineered 7(6):459-477. 10.1080/21655979.2016.1208868 PDF
Foyer CH, Lam H-M, Nguyen HT, Siddique KHM, Varshney RK, Colmer TD, Cowling W, Bramley H, Mori TA, Hodgson JM, Cooper JW, Miller AJ, Kunert K, Vorster J, Cullis C, Ozga JA, Wahlqvist ML, Liang Y, Shou H, Shi K, Yu J, Fodor N, Kaiser BN, Wong F-L, Valliyodan B, Considine MJ. (2016) Neglecting legumes has compromised human health and sustainable food production. Nature Plants 2:16112. 10.1038/nplants.2016.112
Pillay P, Kunert KJ, van Wyk S, Makgopa ME, Cullis CA, Vorster BJ. (2016) Agroinfiltration contributes to VP1 recombinant protein degradation. Bioengineered 7(6):459-477. 10.1080/21655979.2016.1208868
Kunert K, Vorster BJ, Fenta BA, Kibido T, Dionisio G, Foyer CH. (2016) Drought stress responses in soybean roots and nodules. Frontiers in Plant Science 7(1015) 10.3389/fpls.2016.01015 PDF
Sapountzis P, de Verges J, Rousk K, Cilliers M, Vorster BJ, Poulsen M. (2016) Potential for Nitrogen Fixation in the Fungus-Growing Termite Symbiosis. Frontiers in Microbiology 7:1993. 10.3389/fmicb.2016.01993 PDF
Naidoo SIM, Laurie SM, Odeny DA, Vorster BJ, Mphela WM, Greyling MM, Crampton BG. (2016) Genetic analysis of yield and flesh colour in sweetpotato. African Crop Science Journal 24(1):61-73. 10.4314/acsj.v24i1.5 PDF
Sainsbury F, Jutras PV, Vorster J, Goulet MC , Michaud D. (2016) A Chimeric Affinity Tag for Efficient Expression and Chromatographic Purification of Heterologous Proteins from Plants. Frontiers in Plant Science 7:141. 10.3389/fpls.2016.00141
Van Wyk SG, Kunert KJ, Cullis CA, Pillay P, Makgopa ME, Schluter U , Vorster BJ. (2016) The future of cystatin engineering. Plant Science 246:119-127. 10.1016/j.plantsci.2016.02.016 PDF
van Wyk SG, Du Plessis M, Cullis CA, Kunert KJ, Vorster BJ. (2014) Cysteine protease and cystatin expression and activity during soybean nodule development and senescence. BMC Plant Biology 14(294) 10.1186/s12870-014-0294-3
Pillay P, Schlüter U, van Wyk S, Kunert KJ, Vorster BJ. (2014) Proteolysis of recombinant proteins in bioengineered plant cells. Bioengineered 5(1):15-20. 10.4161/bioe.25158
Vorster BJ, Schlüter U, du Plessis M, van Wyk S, Makgopa ME, Ncube I, Quain MD, Kunert K, Foyer CH. (2013) The Cysteine Protease – Cysteine Protease Inhibitor System Explored in Soybean Nodule Development. Agronomy 3(3):550-570. 10.3390/agronomy3030550
Vorster B, Schlüter U, du Plessis M, van Wyk S, Makgopa M, Ncube I, Quain M, Kunert K, Foyer C. (2013) The cysteine protease–cysteine protease inhibitor system explored in soybean nodule development. Agronomy 3(3):550-570. 10.3390/agronomy3030550
Mphangwe NIK, Vorster J, Steyn JM, Nyirenda HE, Taylor NJ, Apostolides Z. (2013) Screening of Tea (Camellia sinensis) for Trait-Associated Molecular Markers. Applied Biochemistry and Biotechnology 171:437-449. 10.1007/s12010-013-0370-4
Vorster BJ, Schlüter U, du Plessis M, van Wyk S, Makgopa M, Ncube I, Quain M, Kunert KJ, Foyer C. (2013) The Cysteine Protease–Cysteine Protease Inhibitor System Explored in Soybean Nodule Development. Agronomy 3(3):550-570.
Kunert KJ, Okole B, Vorster BJ, Brewin NJ, Cullis CA. (2012) A general model for training the next generation of biotechnology entrepreneurs based on recent experience of USA-UK-South Africa collaborations. Journal of Commercial Biotechnology 18:62-66. 10.5912/jcb.525
Pillay P, Kibido T, Du Plessis M, Van der Vyver C, Beyene G, Vorster BJ, Kunert KJ, Schlueter U. (2012) Use of Transgenic Oryzacystatin-I-Expressing Plants Enhances Recombinant Protein Production. Applied Biochemistry and Biotechnology 168(6):1608-1620. 10.1007/s12010-012-9882-6
Sainsbury F, Rheaume A-J, Goulet M-C, Vorster J, Michaud D. (2012) Discrimination of Differentially Inhibited Cysteine Proteases by Activity-Based Profiling Using Cystatin Variants with Tailored Specificities. Journal of Proteome Research 11:5983-5993. 10.1021/pr300699n
Afiukwa CA, Kunert KJ, Vorster J, Cullis CA, Ubi BE. (2011) Microsatellite association with seed protein content and flowering time in Nigerian cowpea cultivars. African Journal of Biotechnology 10:18057-18064. 10.5897/AJB10.2537
Van der Vyver C, Vorster BJ, Kunert KJ, Cullis CA. (2011) Analysis of radiation-induced genome alterations in Vigna unguiculata. Research and Reports in Biology 2:89-99. 10.2147/RRB.S22790
Benchabane M, Schluter U, Vorster J, Goulet M, Michaud D. (2010) Plant cystatins. Biochimie 92(11):1657-1666. 10.1016/j.biochi.2010.06.006
Khalf M, Goulet C, Vorster BJ, Brunelle F, Anguenot R, Fliss I, Michaud D. (2010) Tubers from potato lines expressing a tomato Kunitz protease inhibitor are substantially equivalent to parental and transigenic controls. Plant Biotechnology Journal 8(2):155-169. 10.1111/j.1467-7652.2009.00471.x
Kiggundu A, Muchwezi J, Van der Vyver C, Viljoen A, Vorster BJ, Schluter U, Kunert KJ, Michaud D. (2010) Deleterious effects of plant cystatins against the Banana weevil Cosmopolites sordidus. Archives of Insect Biochemistry and Physiology 73(2):87-105. 10.1002/arch.20342
Schluter U, Benchabane M, Munger A, Kiggundu A, Vorster J, Goulet M, Cloutier C, Michaud D. (2010) Recombinant protease inhibitors for herbivore pest control: a multitrophic perspective. Journal of Experimental Botany 61(15):4169-4183. 10.1093/jxb/erq166
Cullis CA, Vorster BJ, van der Vyver C, Kunert KJ. (2009) Transfer of genetic material between the chloroplast and nucleus: how is it related to stress in plants?. Annals of Botany 103(4):625-633. 10.1093/aob/mcn173
Kunert K, Vorster J, Bester C, Cullis C, Rajasekaran K, Jacks T, Finley J. 2002. DNA microchip technology in the plant tissue culture industry. In: Crop Biotechnology, ACS Symposium Series, Vol. 829. American Chemical Society, USA, pp 86-96.
Vorster BJ, Kunert KJ, Cullis CA. (2002) Use of representational difference analysis for the characterization of sequence differences between date palm varieties. Plant Cell Reports 21(3):271-275. 10.1007/s00299-002-0501-9