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Diplodia pinea diseases

Diplodia pinea (Botryosphaeriaceae) is one of the most important pathogens of pines in South Africa. The fungus has been associated with a number of different disease symptoms, including shoot blight of seedlings and trees (die-back), stem cankers, root diseases and blue stain of timber (Wingfield and Knox-Davies 1980; Swart et al., 1987; Slippers and Wingfield 2007).

Diplodia pinea die-back following hail damage has been known in South Africa since the early 1900's. This serious disease, which accounts for the notoriety of the pathogen worldwide, has led to the restriction of the most susceptible species (Pinus patula, P. pinaster and P. radiata) to areas where hail is infrequent. Pinus patula is still planted in summer rainfall areas and severe losses commonly occur. Although less frequent, hailstorms do occur in areas where P. radiata is planted and these often also lead to substantial losses.

Diplodia pinea exists as a saprophyte on dead bark, cones and needles and is best known as an opportunist that infects wounds on susceptible pines. The fungus can, however, behave as a primary pathogen and infect young, unwounded pine shoots. Moisture is necessary for infection by D. pinea and young shoots become infected when rains coincide with warm temperatures, at the onset of growth. Once established in the shoots, D. pinea moves down the pith, where it can remain dormant. When pines are stressed, the fungus moves out of the pith and causes stem cankers.

A serious root disease of P. elliottii and P. taeda caused by D. pinea has been found in many parts of South Africa. Characteristic symptoms of this disease are dark-blue, radial lesions in young roots which extend to larger roots and into the trunk of diseased trees (Wingfield and Knox-Davies 1980). Needles become chlorotic and are shed. Pinus taeda is apparently most susceptible and the disease is always associated with stress due to factors such as overstocking, drought and plantings on poor sites.

Pitch canker of pines

Pitch canker of pines, caused by the fungus Fusarium circinatum (sexual state Gibberella circinata), has been known in the United States since the early 1900's. In that part of the world, it has caused occasional, yet serious damage to Pinus elliottii, while in California, Pinus radiata, has been seriously damaged by the fungus. Today, the fungus is known to cause nursery problems of Pinus spp. in Spain, Italy, Chile and South Africa, while reports have also been received from Japan.

The pitch canker pathogen was found for the first time in South Africa in the late 1980's where it caused a devastating root disease of P. patula seedlings in a commercial forest nursery in Mpumulanga. The pathogen is believed to have been introduced into the country on contaminated pine seed originating in Central America. The pathogen spread to all commercial pine nurseries within 10 years and is currently the most important nursery disease of Pinus spp. in the country, resulting in losses of up to 50% of P. patula seedlings during epidemics.

Significant losses, of especially P. patula, are experienced annually during establishment, as a result of plants dying from F. circinatum infection shortly after transplanting. It has been calculated that ~40% of plants moving to the field are asymptomatic, but develop disease soon after transplanting, resulting in high levels of blanking. According to a recent survey, 75% more seedlings are required to establish a P. patula stand as a result of F. circinatum infection of plants (Andrew Morris, Sappi 2010).

In recent years pitch canker disease on mature trees has been found in the Western, Southern and North Eastern Cape Provinces. The disease has resulted in the death of P. radiata, P. patula and P. greggii. It has also been shown to be associated with damage to trees caused by the weevil Pissodes nemorensis (Coutinho et al. 2007).

Chrysoporthe (Cryphonectria) canker of eucalypts

Chrysoporthe canker of eucalypts in South Africa is caused by the fungus Chrysoporthe austroafricana. The same disease in South America and Asia is caused respectively by Chr. cubensis (previously known as Cryphonectria cubensis) and Chr. deuterocubensis (previously known as C. cubensis). Although once considered the most serious disease of eucalypts in the world, the disease in many countries has been managed successfully through aggressive breeding and selection programmes and the deployment of disease tolerant clones (Gryzenhout et al., 2009).

In South Africa, disease of eucalypts caused by Chr. austroafricana is restricted to sub-tropical areas of the country. Although it commonly causes basal cankers on large trees, the most serious damage is to young E. grandis that become girdled at the base. Symptoms of the disease on young trees are a general wilting, and trees dying from Cryphonectria canker can easily be confused with those infected with Pythium sp. in Zululand. It is usually possible to identify trees infected with Chr. austroafricana by the presence of long-necked dark pycnidia (flask-shaped structures that produce and contain asexual spores) with orange spore masses at their apices, on the surface of the cankers. These can, however, be absent owing to unfavourable conditions for their production or because they have been consumed by insects. As is true in the case of most diseases, laboratory diagnosis is usually necessary.

Both Chr. cubensis and Chr. deuterocubensis have been introduced to the African continent, but as yet have not been found in South Africa. Chrysoporthe deuterocubensis, however, has been identified in central Mozambique and it is likely only a matter of time before it, or Chr. cubensis, appears in the country. The consequences of the introduction of these species into the country is not clear, although studies to date suggest that they are not as virulent as Chr. austroafricana. Care should, however, be taken not to move infected material into the country.

Botryosphaeria canker and die-back of eucalypts and black wattle

Fungi classified in the Botryosphaeriaceae are commonly associated with disease symptoms on eucalypts, Acacia mearnsii and Pinus spp. In the previous century, the disease of eucalypts and wattle was ascribed to Botryosphaeria dothidea. Research has, however, shown that Botryosphaeria canker is associated with numerous fungal species within a larger group, known as the Botryosphaeriaceae. This includes species of Neofusicoccum, Lasiodiplodia and Botryosphaeria.

Fungi in the Botryosphaeriaceae cause diseases characterized by stem cankers, shoot and branch die-back as well as root and root collar disease. Indeed, some of the most serious losses in plantations of eucalypts (and wattle) have been associated with the Botryosphaeriaceae. These fungi are opportunistic pathogens (often with a latent asymptomatic phase) and their importance is often underestimated. In terms of South African forestry, this fact is well illustrated by the importance of the closely related and opportunistic D. pinea on pines. Factors associated with infection of eucalypts and black wattle by fungi in the Botryosphaeriaceae include damage to growing tips by hot and cold winds or late frost, mechanical damage through hail, insect feeding or pruning and drought stress. One or more of these conditions commonly occur in local plantations and it is not surprising that extensive losses have been associated with these fungi (Slippers and Wingfield 2007).

Holocryphia (Endothia) canker of eucalypts

Holocryphia eucalypti (previously known as Endothia gyrosa and Cryphonectria eucalypti) causes a bark canker disease of eucalypts in many parts of South Africa and Swaziland (Gryzenhout et al., 2009). The pathogen is common on most eucalypt species in the country, causing superficial cracking of the bark. In some cases, however, the pathogen appears to penetrate the bark and infect the cambium. Infection of eucalypts appears to be dependent on two factors that can be, but are not necessarily, related. These include stress and inherent susceptibility of the trees. Cankers are most serious in areas affected by drought stress. However, certain species and clones of E. grandis, planted on what are considered to be excellent sites, can also be severely affected.

In Australia, H. eucalypti has been associated with the death of eucalypts, and is considered an important pathogen of these trees. Australia is believed to be the origin of H. eucalypti and it has been shown that the fungus in South Africa is represented by a limited diversity, indicative of an introduced pathogen.

Coniothyrium canker of eucalypts

A serious canker disease of Eucalyptus species and hybrid clones, in the Zululand region of KwaZulu-Natal is caused by the fungus first known as Coniothyrium zuluense. Significant taxonomic revision of this pathogen and the family of fungi it belongs to has resulted in several name changes, with the most recent suggesting that it should be known as Teratosphaeria zuluense. It has also been shown that a second species, T. gauchensis occurs in South America and Ethiopia, causing a similar disease of eucalypts in those countries (Cortinas et al., 2006).

Coniothyrium canker is characterised by measle-like lesions on the young green stem tissue associated with the current year's growth. As the infection progresses, lesions become confluent and cracks that exude kino, develop in the necrotic tissue. Infection also results in severe malformation of the stems which become swollen and spindle-shaped in the areas of infection. New infections occur annually on new growth, while those from previous years continue to cause malformation and disease. Cankers, therefore, commonly occur in discrete zones at various heights on the stems of affected trees.

Susceptibility to Coniothyrium canker is variable on different clones. The most susceptible clones eventually cease to grow and their tops begin to die. It is unlikely that such clones can be successfully propagated commercially.

Pink disease of eucalypts

Erythricium salmonicolor (Corticium salmonicolor, Phanerochaeta salmonicolor) is a well-known canker pathogen of woody plants in tropical parts of the world and the associated disease is commonly known as pink disease. The disease has been known in South Africa for many years, particularly on deciduous fruit trees. It has been found affecting eucalypts, Acacia mearnsii and Podocarpus spp. in the KZN Midlands of South Africa, causing severe stem cankers and stem breakage on eucalypts and A. mearnsii. It has also been found causing branch and top death of Podocarpus spp. and Ekebergia capensis in the Sabie and Howick areas (Roux and Coetzee 2005).

Pink disease derives its name from the pink to salmon-coloured superficial fungal growth that occurs on the surface of cankers. Early in the infection process, a white mycelial mat develops on the bark surface. Cankers develop behind the leading edge of the mycelium where the cambium is penetrated by the fungus. The fungus requires relative humidity of more than 90 % for successful germination and infection to take place.

Cytospora (Valsa) canker of eucalypts

Three species of Cytospora, C. australiae, C. eucalypticola (Teleomorph: Valsa fabianae) and C. eucalyptina, have been reported associated with cankers on eucalypts in South Africa (Adams et al., 2005). In recent years, only C. eucalypticola has been found during routine surveys, and it has been shown that reports of C. australiae actually represent other species. Cytospora eucalypticola, which is also known on eucalypts elsewhere in the world, appears to be only mildly pathogenic. It is usually isolated from severely stressed trees and on dying tissue. Recent research has shown that the fungus is an endophyte that is present in asymptomatic tissue. It presumably then proliferates when stress conditions appear.

Bacterial wilt of eucalypts

Bacterial wilt of eucalypts is caused by the bacterium Ralstonia solanacearum. The disease is known in countries such as Brazil, China and Uganda and has been recorded from Eucalyptus clones in the Zululand forestry area of South Africa (Coutinho et al., 2000). The disease has, thus far, been found only in a small number of plantations where it has caused rapid wilt and death of trees at the onset of hot dry conditions in summer. Wilt is initially seen on single branches. Symptoms then spread rapidly to other branches and eventually the entire tree. The xylem of these trees show a brown discoloration and bacteria ooze from damaged wood almost immediately after cutting. When infected branches or stems are placed in water, a bacterial stream can easily be seen emerging from cut ends and the water soon becomes murky due to contamination with bacterial cells. There is strong emerging evidence to show that the bacterium is only serious on trees that are severely stressed and particularly those with root knots.

Black butt disease of wattle

Black discoloration of the bases of wattle trees is a common symptom wherever these trees are grown. The cause of this disease is complex and isolations from diseased tissue have yielded a number of opportunistic pathogens. It is likely that this symptom represents a complex disease situation without any single pathogen being responsible for the problem. It has also been suggested that gummosis at the bases of trees caused by Phytophthora nicotianae might be an initial stage of this disease, providing entry sites for opportunistic pathogens. Early infections by basidiomycetes and Phytophthora spp. might also provide entry wounds for the wilt pathogen, Ceratocystis albifundus, as well as other pathogens. This then leads to the spread of the black lesions to cover the entire tree in severe cases (Roux 2002).

Ceratocystis wilt of wattle

Ceratocystis wilt (wattle wilt) is caused by the fungus Ceratocystis albifundus and is the most serious disease of Acacia mearnsii in Africa. The disease occurs throughout South Africa, wherever A. mearnsii and A. decurrens occurs (Roux 2002).

Ceratocystis albifundus requires wounds to infect trees. Thus, wounds made when clearing plantations of weeds, or when stems of trees are 'singled' can lead to the onset of disease. Hail damage also provides excellent points of entry for C. albifundus and many other pathogens. Nitidulidae and other insects visiting wounds are the main means of spread of the pathogen. Once infection has occurred, the fungus moves rapidly within trees causing gum exudation from the stems. Wood becomes discoloured in a streaked pattern. Infe

New Publications

Twiddy D, Fouché A, Akinsanmi OA, Fourie G. (2024) Biology and pathogenicity of fungi causing husk rot of macadamia in South Africa. European Journal of Plant Pathology :1-18. 10.1007/s10658-024-02915-z
Visagie CM, Yilmaz N, Allison JD, Barreto RW, Boekhout T, Boers J, Delgado MA, Dewing C, Fitza KNE, Furtado ECA, Gaya E, Hill R, Hobden A, Hu DM, Hülsewig T, Khonsanit A, Luangsa-Ard JJ, Mthembu A, Pereira CM, Price J-L, Pringle A, Qikani N, Sandoval-Denis M, Shumacher RK, Seifert KA, Slippers B, Tennakoon DS, Thanakitpipattana D, vanVuuren N, Groenewald JZ, Crous PW. (2024) New and Interesting Fungi. 7.. Fungal Systematics and Evolution 13:441–494. 10.3114/fuse.2024.13.12 PDF
Coutinho TA, Carstensen G, Venter SN, Chen SF, Tarigan M, Wingfield MJ. (2024) Bacterial community in apparently healthy and asymptomatic Eucalyptus trees and those with symptoms of bacterial wilt. Journal of Plant Pathology 10.1007/s42161-024-01697-x
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Visagie CM, Cruywagen EM, Duong TA. (2024) A new Paecilomyces from wooden utility poles in South Africa. Fungal Systematics and Evolution 13:163–181. 10.3114/fuse.2024.13.10
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Ceriani C, Wingfield MJ, Fru F, van Wyk S, Rodas C, Wingfield BD, Steenkamp ET. (2024) Clonality and limited population diversity of Fusarium circinatum in Colombia. Forest Pathology 54(3):e12864. 10.1111/efp.12864
Jami F, Duma S, Fourie G, Schoeman M. (2024) First report of Botrytis cinerea causing flower blight on macadamia in South Africa. Journal of Phytopathology 72:e13325.:1-4. 10.1111/jph.13325
Paap T, Balocchi F, Burgess TI, Bose T, Wingfield MJ. (2024) A diverse range of Phytophthora species from botanical gardens in South Africa, including the novel Clade 5 species, Phytophthora mammiformis sp. nov.. Fungal Systematics and Evolution 13:111-122. 10.3114/fuse.2024.13.05
Ramaswe JB, Steenkamp ET, De Vos L, Fru FF, Adegeye OO, Wingfield BD. (2024) Sex pheromone receptor Ste2 orchestrates chemotropic growth towards pine root extracts in the pitch canker pathogen Fusarium circinatum. Pathogens 13:425. 10.3390/pathogens13050425
Midgley KA, van den Berg N, Swart V. (2024) Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection. BMC Genomics 25:435. 10.1186/s12864-024-10358-3
Marais I, Buitendag C, Duong TA, Crampton BG, Theron J, Kidanemarium D, Berger DK. (2024) Double-stranded RNA uptake for the control of the maize pathogen Cercospora zeina. Plant Pathology 73(6):1480-1490. 10.1111/ppa.13909
van Heerden A, Pham NQ, Wingfield BD, Wingfield MJ, Muro Abad JI, Durán A, Wilken PM. (2024) LAMP assay to detect Elsinoë necatrix; an important Eucalyptus shoot and leaf pathogen. Plant Disease 10.1094/PDIS-01-24-0086-RE
Silva GA, Oliveira MES, Rêgo GMS, Wingfield BD, Wingfield MJ, Ferreira MA. (2024) Chrysoporthe brasiliensis sp. nov. pathogenic to Melastomataceae in southeast Brazil. Fungal Biology 10.1016/j.funbio.2024.04.001
Fick A, Swart V, Bombarely A, van den Berg N. (2024) Comparative transcriptional analysis of Persea americana MYB, WRKY and AP2/ERF transcription factors following Phytophthora cinnamomi infection. Molecular Plant Pathology 25(4):e13453. 10.1111/mpp.13453 PDF
Van Lill M, Venter SN, Muema EK, Palmer M., Beukes CW, Chan WY, Steenkamp ET. (2024) SeqCode facilitates naming of South African rhizobia left in limbo. Systematics and Applied Microbiology 47(2-3):126504. 10.1016/j.syapm.2024.126504 PDF
Visagie CM, Meyer H, Yilmaz N. (2024) Maize–Fusarium associations and their mycotoxins: Insights from South Africa. Fungal Biology 10.1016/j.funbio.2024.03.009
Elisa P, Allison J, Hurley BP, Slippers B, Fourie G. (2024) Lethal and sublethal effects of insecticides on Bathycoelia distincta (Heteroptera: Pentatomidae). African Entomology 32: e16992:1-9. 10.17159/2254-8854/2024/a16992
Pietersen G, Morgan S, Read DA. (2024) First report of Citrus concave gum-associated virus (CCGaV) on apple (Malus spp.) in South Africa. Journal of Plant Pathology 10.1007/s42161-024-01629-9
Gush S, Lebre P, Coutinho TA, Cowan DA, van der Waals JE. (2024) Disentangling shifts in the soil microbiome of potatoes infected with Rhizoctonia solani Anastomosis Group 3-PT in search of potential biocontrol agents. Phytobiomes Journal 10.1094/PBIOMES-06-23-0046-R
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Bornman S, Thomas C, Ntladi S, Wilken PM. (2024) Sclerotinia sclerotiorum is the causal agent of Sclerotinia stem rot on peanut (groundnut) in South Africa. Australasian Plant Disease Notes 19 10.1007/s13314-024-00537-2
Abkallo HM, Arbuthnot P, Auer TO, Berger DK, Burger J, Chakauya E, Concordet J-P, Diabate A, Di Donato V, Groenewald J-H, Guindo A, Koekemoer LL, Nazare F, Nolan T, Okumu F, Orefuwa E, Paemka L, Prieto-Godino L, Runo S, Sadler M, Tesfaye K, Tripathi L, Wondji C. (2024) Making genome editing a success story in Africa. Nature Biotechnology :1-4. https://rdcu.be/dBJUa
Robert R, Robberste N, Thompson GD, Read DA. (2024) Characterization of macadamia ringspot‑associated virus, a novel Orthotospovirus associated with Macadamia integrifolia in South Africa. European Journal of Plant Pathology 10.1007/s10658-024-02832-1 PDF
Hiroyuki S, Marincowitz S, Roux J, Paap T, Wingfield BD, Wingfield MJ. (2024) A new genus and species of Cryphonectriaceae causing stem cankers on plantation eucalypts in South Africa. Plant Pathology :1-14. 10.1111/ppa.13883 PDF
Strydom RF, Wilson CR, Tegg RS, Balendres MA, van der Waals JE. (2024) Advancements in Spongospora subterranea: Current knnowledge, management strategies, and research gaps. Potato Research 10.1007/s11540-024-09701-8
Mapfumo P, Buthelezi S, Archer E, Swanevelder DZH, Wilken PM, Creux N. (2024) In-field climatic factors driving Sclerotinia head rot progression across different sunflower planting dates. Plant Pathology 10.1111/ppa.13873
Price J-L, Visagie CM, Meyer H, Yilmaz N. (2024) Fungal species and mycotoxins Associated with Maize ear rots collected from the Eastern Cape in South Africa. Toxins 16:95. 10.3390/toxins16020095
Tarigan M, Wingfield MJ, Jami F, Marpaung YMAN, Duran A, Pham NQ. (2024) Pathogenicity of Chrysoporthe deuterocubensis on eucalypts in Indonesia. Southern Forests: a Journal of Forest Science 10.2989/20702620.2023.2279054
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MISEV Consortium, Motaung T. (2024) Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of Extracellular Vesicles 13(2):e12404. 10.1002/jev2.12404
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Francinah M. Ratsoma, Nthabiseng Z. Mokoena, Quentin C. Santana, Brenda D. Wingfield, Emma T. Steenkamp, Thabiso E. Motaung. (2024) Characterization of the Fusarium circinatum biofilm environmental response role. Journal of Basic Microbiology 00(00):1-16. 10.1002/jobm.202300536
Morrison EW, Duong TA, Garnas JR. (2024) A high-quality draft genome sequence of Neonectria faginata, causative agent of beech bark disease of Fagus grandifolia. Microbiology Resource Announcements 10.1128/mra.01048-23
Roberts E, Paap T, Roets F. (2024) Chemical control of the Polyphagous Shothole Borer beetle (PSHB, Euwallacea fornicatus) and Fusarium euwallaceae in American sweetgum (Liquidambar styraciflua). Journal of Plant Pathology 10.1007/s42161-023-01583-y
Fitza KNE, Allison J, Slippers B, Chingandu N, Reed SE. (2024) Diversity and potential sources of introduction of the Beech leaf nematode (Litylenchus crenatae< mccannii) to Ontario, Canada. Canadian Journal of Plant Pathology
Engelbrecht K, Raubenheimer I, Paap T, Neethling E, Roets F. (2024) Detection of Fusarium euwallaceae and its vector Euwallacea fornicatus on Pear (Pyrus communis) and in deciduous fruit orchards in South Africa. Australasian Plant Disease Notes 19(1) 10.1007/s13314-023-00524-z PDF
Visagie CM, Meijer M, Kraak B, Groenewald M, Houbraken J, Theelen B, Vorst Y, Boekhout T. (2024) Blastobotrys nigripullensis, a new yeast species isolated from a fungal outbreak on an ancient Roman shipwreck in the Netherlands. Antonie Van Leeuwenhoek 117:22. 10.1007/s10482-023-01898-x
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Chen BY, Wu WX, Chen SF. (2024) Wide distribution of Teratosphaeria epicoccoides and T. destructans associated with diseased Eucalyptus leaves in plantations in Southern China. Microorganisms 12:129. 10.3390/microorganisms12010129
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Wingfield MJ, Pham NQ, Marincowitz S, Duong TA, Wingfield BD, Wilson AM. (2024) Blast from the past: a study of decades-old fungal cultures resolves a long-standing tree disease mystery. Journal of Plant Pathology 106:377–384. 10.1007/s42161-023-01502-1
Nganso BT, Soroker V, Osabutey AF, Pirk CWW, Johansson T, Elie N, Otieno-Ayayo ZN, Ibrahim MM, Ndungu NN, Ayalew W, Wubie AJ, Taboue GCT, Fameni ST, Bobadoye BO, Assefa F, Subramanian S. (2024) Best practices for colony management: a neglected aspect for improving honey bee colony health and productivity in Africa. Journal of Apicultural Research :1-18. 10.1080/00218839.2024.2308418
Yusuf A, Pirk C, Crewe R. (2024) A Hitchhiker’s Ride: The Honey Bee Louse Braula Coeca (Diptera: Braulidae) Selects its Host by Eavesdropping. Journal of Chemical Ecology 10.1007/s10886-024-01481-2
Crous PW, Costa MM, Kandemir H, Vermaas M, Vu D, Zhao L, Arumugam E, Flakus A, Jurjević Ž, Kaliyaperumal M, Mahadevakumar S, Murugadoss R, Shivas RG, Tan YP, Wingfield MJ, Abell SE, Marney TS, Danteswari C, Darmostuk V, Denchev CM, Denchev TT, Gené J, Etayo J, Gunaseelan S, Hubka V, Illescas T, Jansen GM, Kezo K, Kumar S, Larsson E, Mufeeda KT, Piatek M, Rodriguez-Flakus P, Sarma PVSRN, Stryjak-Bokacka M, Torres-Garcia D, Vauras J, Acal DA, Akulov A, Alhudaib K, Asif M, Balashov S, Baral H-O, Baturo-Cieśniewska A, Begerow D, Beja-Perreira A, Bianchinotti MV, Bilański P, Chandranayaka S, Chellappan N, Cowan DA, Custódio FA, Czachura P, Delgado G, De Silva NI, Dijksterhuis J, Dueñas M, Eisvand P, Fachada V, Fournier J, Fritsche Y, Fuljer F, Ganga KGG, Guerra MP, Hansen K, Hywel-Jones N, Ismail AM, Jacobs CR, Jankowiak R, Karich A, Kemler M, Kisło K, Klofac W, Krisai-Greilhuber I, Latha KPD, Lebeuf R, Lopes ME, Lumyong S, Maciá-Vicente JG, Maggs-Kölling G, Magistà D, Manimohan P, Martín MP, Mazur E, Mehrabi-Koushki M, Miller AN, Mombert A, Ossowska EA, Patejuk K, Pereira OL, Piskorski S, Plaza M, Podile AR, Polhorsky A, Pusz W, Raza W, Ruszkiewicz-Michalska M, Saba M, Sánchez RM, Singh R, Śliwa L, Smith ME, Stefenon VM, Strašiftáková D, Suwannarach N, Szczepańska K, Telleria MT, Tennakoon DS, Thines M, Thorn RG, Urbaniak J, van der Vegte M, Vasan V, Vila-Vićosa C, Voglmayr H, Wrzosek M, Zappelini J, Groenewald JZ. (2023) Fungal Planet description sheets: 1550-1613. Persoonia 51:280-417. 10.3767/persoonia.2023.51.08
Wilken PM, Lane FA, Steenkamp ET, Wingfield MJ, Wingfield BD. (2023) Unidirectional mating-type switching is underpinned by a conserved MAT1 locus architecture. Fungal genetics and Biology (103859) 10.1016/j.fgb.2023.103859
Schertler A, Thines M, Dawson W, van Kleunen M, Kreft H, Pergl J, Pyšek P, Weigelt P, Winter M, Seebens H, Wingfield MJ, Reino L, Lenzner B, Dellinger S, Moser D, Bufford JL, Ghelardini L, Santini A, Capinha C, Monteiro M, Essl F. (2023) Biogeography and global flows of 100 major alien fungal and fungus-like oomycete pathogens. Journal of Biogeography 10.1111/jbi.14755
van der Merwe E, Slippers B, Dittrich-Schröder G. (2023) Mechanical egg activation and rearing of first instar larvae of Sirex noctilio (Hymenoptera: Siricidae). Insects 14(12):931. https://www.mdpi.com/2075-4450/14/12/931
Wingfield MJ, Marincowitz S, Barnes I, Tarigan M, Solís M, Duran A, Pham NQ. (2023) First report of phyllode rust on Acacia crassicarpa outside its native range. Forest Pathology 10.1111/efp.12839
Fourie A, Venter S, Slippers B, Fourie G. (2023) Pantoea bathycoeliae sp. nov and a Sodalis sp. are core gut microbiome symbionts of the two-spotted stink bug. Frontiers in Microbiology :1-19. 10.3389/fmicb.2023.1284397 PDF
Rakubu IL, Katumanyane A, Hurley BP. (2023) Host-foraging strategies of five local entomopathogenic nematode species in South Africa. Crop Protection 176:106525. 10.1016/j.cropro.2023.106525
Barten H, Schröder ML, Slippers B, Howe AG, Lawson SA, Hurley BP. (2023) Reproductive compatibility of a newly imported Australian population of the biocontrol agent Anaphes nitens with an existing South African population. Biological Control 187:105403. 10.1016/j.biocontrol.2023.105403 PDF
Anbu SP, Swart V, van den Berg N. (2023) Unmasking the invaders: NLR-mal function in plant defense. Frontiers in Plant Science 14:1307294. 10.3389/fpls.2023.1307294 PDF
Pham NQ, Duong TA, Wingfield BD, Barnes I, Duran A, Wingfield MJ. (2023) Characterisation of the mating-type loci in species of Elsinoe causing scab diseases. Fungal Biology 127:1484–1490. 10.1016/j.funbio.2023.11.003
Wu WX, Li WW, Liu FF, Chen SF. (2023) Evidence of High Genetic Diversity and Differences in the Population Diversity of the Eucalyptus Leaf Blight Pathogen Calonectria pseudoreteaudii from Diseased Leaves and Soil in a Plantation in Guangxi, China. Microorganisms (11) 10.3390/microorganisms11112785 PDF
Backer R, Naidoo S, van den Berg N. (2023) The expression of the NPR1-dependent defense response pathway genes in Persea americana (Mill.) following infection with Phytophthora cinnamomi. BMC Plant Biology 23(1):548. 10.1186/s12870-023-04541-z PDF
Rakubu IL, Katumanyane A, Hurley BP. (2023) Screening five local entomopathogenic nematode species for their virulence against pupae of the Eucalyptus snout beetle, Gonipterus sp. n. 2, under laboratory conditions . Crop Protection 176:106500. 10.1016/j.cropro.2023.106500
Omondi DO, Dida MM, Berger DK, Beyene Y, Nsibo DL, Juma C, Mahabaleswara SL, Gowda M. (2023) Combination of Linkage and Association Mapping with Genomic Prediction to Infer QTL Regions Associated with Gray Leaf Spot and Northern Corn Leaf Blight Resistance in Tropical Maize. Frontiers in Genetics 14:1-16. 10.3389/fgene.2023.1282673
Granados GM, Rodas CA, Vivas M, Wingfield MJ, Barnes I. (2023) Patterns of Dothistroma septosporum conidial dispersal in Colombian Pinus tecunumanii plantations. Forest Pathology (00):e12834. 10.1111/efp.12834
Bose T, Hammerbacher A, Spies CFJ, Coutinho TA. (2023) Phytophthora: an underestimated threat to agriculture, forestry, and natural ecosystems in sub-Saharan Africa. Mycological Progress 22:78. 10.1007/s11557-023-01926-0
Robert Mangani, Kpoti M. Gunn, Nicky Creux. (2023) Projecting the effect of climate change on planting date and cultivar choice for South African dryland maize production. Agricultural and Forest Meteorology 341(15):109695. 10.1016/j.agrformet.2023.109695
Wilson AM, Coetzee MPA, Wingfield MJ, Wingfield BD. (2023) Needles in fungal haystacks: Discovery of a putative a-factor pheromone and a unique mating strategy in the Leotiomycetes. PLOS One 18(10):e0292619. 10.1371/ journal.pone.0292619 PDF
Visagie CM, Magistà D, Ferrara M, Balocchi F, Duong TA, Eichmeier A, Gramaje D, Aylward J, Baker SE, Barnes I, Calhoun S, De Angelis M, Frisvad JC, Hakalova E, Hayes RD, Houbraken J, Grigoriev IV, LaButti K, Leal C, Lipzen C, Ng V, Pangilinan J, Pecenka J, Perrone G, Piso A, Savage E, Spetik E, Wingfield MJ, Zhang Y, Wingfield BD. (2023) IMA genome-F18 - The reidentification of Penicillium genomes available in the NCBI and draft genomes for Penicillium species from dry cured meat, Penicillium biforme, P. brevicompactum, P. solitum, and P. cvjetkovicii, Pewenomyces kutranfy, Pew. lalenivora, Pew. tapulicola, Pew. kalosus, Teratosphaeria carnegiei, and Trichoderma atroviridae SC1. IMA Fungus 14(21) 10.1186/s43008-023-00121-w
Welgemoed T, Duong TA, Barnes I, Stukenbrock EH, Berger DK. (2023) Population genomic analyses suggest recent dispersal events of the pathogen Cercospora zeina into East and Southern African maize cropping systems. G3 Genes|Genomes|Genetics 10.1093/g3journal/jkad214
Tarigan M, Wingfield MJ, Marpaung YMAN, Duran A, Pham NQ. (2023) Quambalaria eucalypti found on Eucalyptus in Indonesia. Forest Pathology :e12829. 10.1111/efp.12829 PDF
Narh Mensah DL , Wingfield BD, Coetzee MPA. (2023) A practical approach to genome assembly and annotation of Basidiomycota using the example of Armillaria. BioTechniques 10.2144/btn-2023-0023
Avontuur JR, Wilken PM, Palmer M, Coetzee MPA, Stępkowski T, Venter SN, Steenkamp ET. (2023) Complex evolutionary history of photosynthesis in Bradyrhizobium. Microbial Genomics 9(9) 10.1099/mgen.0.001105
Fourie G, Mkhize T, Twiddy D. (2023) Bark and Ambrosia beetles and their fungal symbionts in macadamia orchards in South Africa. SAMAC Journal :78-79.
Twiddy D, Akinsanmi F, Fourie G. (2023) Towards management of macadamia nut diseases in South Africa. SAMAC Journal :80-81.
Fourie G. (2023) Disease diagnostic clinic and field extension: a service to the macadamia industry. SAMAC Journal :96-97.
Moffat R, Hurley BP, Fourie G. (2023) Biology of the invasive macadamia felted coccid, Acanthococcus ironsidei Williams (Hemiptera: Eriococcidae) and potential native enemies as biological control agents. SAMAC Journal :82-83.
Smith A, Fourie G. (2023) The Macadamia nut borer genome: What are the benefits?. SAMAC Journal :84.
Oliveira LS, Saha MA, Jami F, Asfa R, Maretha MV, Sipayung J, Tarigan M, Duran A. (2023) Identification, pathogenicity and control of powdery mildew caused by Erysiphe elevata on Eucalyptus pellita in Sumatra, Indonesia. . Forest Pathology 53(5):e12825. 10.1111/efp.12825
Anneri Lötter, Tuan A Duong, Julia Candotti, Eshschar Mizrachi, Jill L Wegrzyn, Alexander A Myburg. (2023) Haplogenome assembly reveals structural variation in Eucalyptus interspecific hybrids. GigaScience 12 10.1093/gigascience/giad064
Balocchi F, Barnes I, Wingfield MJ, Ahumada R, Visagie CM. (2023) New Resinogalea species from Araucaria araucana resin in Chile and reclassification of the genus in the Cryptocaliciomycetidae. IMA Fungus 14 10.1186/s43008-023-00122-9 PDF
Claassens R, Venter SN, Beukes CW, Stępkowski T, Chan WY, Steenkamp ET. (2023) Bradyrhizobium xenonodulans sp. nov. isolated from nodules of Australian Acacia species invasive to South Africa. Systematic and Applied Microbiology 10.1016/j.syapm.2023.126452
Johannes Joubert, Benice Sivparsad, Michelle Schroder, Ilaria Germishuizen, Jingyuan Chen, Brett Hurley, Jeremy Allison, Almuth Hammerbacher. (2023) Susceptibility of Eucalyptus trees to defoliation by the Eucalyptus snout beetle, Gonipterus sp. n. 2, is enhanced by high foliar contents of 1,8-cineole, oxalic acid and sucrose and low contents of palmitic and shikimic acid. Plant, Cell & Environment 10.1111/pce.14696 PDF
Pham NQ, Wingfield MJ, Marincowitz S, Tanga AA, Tiki KR, Kassie WB, Hurley BP, Germishuizen I, Lawson SA, Healey MA, Wondafrash M. (2023) First report of the wattle rust pathogen, Uromycladium acaciae (Raveneliaceae, Pucciniales) in Ethiopia. Forestry: An International Journal of Forest Research :1–8. 10.1093/forestry/cpad040
Swanepoel S, Visser EA, Shuey SS, Naidoo S. (2023) The In Planta Gene Expression of Austropuccinia psidii in Resistant and Susceptible Eucalyptus grandis. Phytopathology 113(6):1066-1076. 10.1094/PHYTO-07-22-0257-R PDF
Jami F, Botha W. (2023) Pythium and related species on blueberry plants in South Africa. Journal of Phytopathology 10.1111/jph.13213
Paap T, Marincowitz S, Pham NQ, Roets F, Basson RJ, Wingfield BD, Oberlander K, Wingfield MJ. (2023) A novel species of Microsphaeropsis causing cankers on Rafnia amplexicaulis in South Africa. Fungal Systematics and Evolution 12:73-80. 10.3114/fuse.2023.12.05 PDF
Marincowitz S, Pham NQ, Wingfield BD, Roets F, Wingfield MJ. (2023) Microfungi associated with dying Euphorbia mauritanica in South Africa and their relative pathogenicity. Fungal Systematics and Evolution 12:59-71. 10.3114/fuse.2023.12.04 PDF
Wu WX. (2023) Wide distribution and intraspecies diversity in the pathogenicity of Calonectria in soil from Eucalyptus plantations in southern Guangxi of China. Journal of Fungi 9(8):802. PDF
Wu WX, Chen SF. (2023) Wide distribution and intraspecies diversity in the pathogenicity of Calonectria in soil from Eucalyptus plantations in southern Guangxi of China. Journal of Fungi 9(8):802.
Kooverjee BB, Soma P, van der Nest MA, Scholtz MM, Neser FWC. (2023) Copy Number Variation Discovery in South African Nguni-Sired and Bonsmara-Sired Crossbred Cattle. Animals 13(15):2513. 10.3390/ani13152513
Makunde PT, Joubert JC, Slippers B, Hurley BP, Hammerbacher A. (2023) Leaf surface traits may influence host specificity in psyllids of Eucalyptus, Spondyliaspis cf. plicatuloides (Froggatt) and Glycaspis brimblecombei Moore (Hemiptera: Aphalaridae). Chemoecology 33:83-98. 10.1007/s00049-023-00387-x
Harris MA, Slippers B, Kemler M, Greve M. (2023) Opportunities for diversified usage of metabarcoding data for fungal biogeography through increased metadata quality. Fungal Biology Reviews 46:100329. 10.1016/j.fbr.2023.100329
Franić I, Allan E, Prospero S, ..., Paap T, et al.. (2023) Climate, host and geography shape insect and fungal communities of trees. Scientific Reports 13:1-13. 10.1038/s41598-023-36795-w
Katumanyane A, Slippers B, Wondafrash M, Malan AP, Hurley BP. (2023) Natural infection of white grubs (Coleoptera: Scarabaeidae) with entomopathogenic nematodes in the KwaZulu-Natal province of South Africa. Journal of Helminthology 97 10.1017/S0022149X23000378
Crous P, Pancorbo F, Luangsa-ard J, Larsson E, Esteve-Raventós F, Bishop-Hurley S, Tan Y, Shivas R, Osieck E, Balashov S. (2023) Fungal Planet description sheets: 1478–1549. Persoonia 50:158-310. 10.3767/persoonia.2023.50.05
Crous P, Cowan D, Cruz R, Czachura P, De la Peña-Lastra S, Dovana F, Drury B, Fell J, Flakus A, Fotedar R, Jurjević , Chandranayaka S, Boekhout T, Osieck E, Shivas R, Tan Y, Bishop-Hurley S, Esteve-Raventós F, Larsson E, Luangsa-ard J, Pancorbo F, Balashov S, Baseia I, Kolecka A. (2023) Fungal Planet description sheets: 1478–1549 . Persoonia 50:58 – 310. 10.3767/persoonia.2023.50.05
Nkomo T, Hammerbacher A, Wingfield BD, Bose T. (2023) Fungal Planet description sheets: 1478–1549 (Description of Tulasnella epidendrea). Persoonia - Molecular Phylogeny and Evolution of Fungi 50:290-291. 10.3767/persoonia.2023.50.05
Read DA, Thompson GD, Swanevelder DZH, Pietersen G. (2023) Metaviromic Characterization of Betaflexivirus Populations Associated with a Vitis cultivar Collection in South Africa. Viruses 15(7):1474. 10.3390/v15071474
Li WW, Chen SF, Wingfield MJ, Duong TA. (2023) Calonectria species associated with diseased leaves and soils in southern China Eucalyptus plantations. Phytopathology Research 5(29) 10.1186/s42483-023-00183-z
Roux J, Wingfield MJ, Marincowitz S, Solís M, Phungula S, Pham NQ. (2023) Eucalyptus scab and shoot malformation: a new disease in South Africa caused by a novel species, Elsinoe masingae. Forestry: An International Journal of Forest Research 10.1093/forestry/cpad031
Chepsergon J, Moleleki LN. (2023) “Order from disordered”: Potential role of intrinsically disordered regions in phytopathogenic oomycete intracellular effector proteins. Current Opinion in Plant Biology 75:102402. 10.1016/j.pbi.2023.102402
van der Walt D, Steenkamp ET, Wingfield BD, Wilken PM. (2023) Evidence of Biparental Mitochondrial Inheritance from Self-Fertile Crosses between Closely Related Species of Ceratocystis. Journal of Fungi 9(6):686. 10.3390/jof9060686 PDF
Barnes I, Abdul Rauf MR, Fourie A, Japarudin Y, Wingfield MJ. (2023) Ceratocystis manginecans and not C. fimbriata a threat to propagated Acacia spp. in Sabah, Malaysia. Journal of Tropical Forest Science 35:16-26. 10.26525/jtfs2023.35S.SI.16