Ms Nare Ngoepe

PhD student


Biochemistry, Genetics and Microbiology
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I joined FABI in 2012 during my Masters degree which I obtained in Biotechnology from the Plant Sciences Department, supervised by Dr Bridget Crampton. My project was titled "Genotyping-by-Sequencing of sweet-stem and grain sorghum for linkage mapping". The main aim of my project was to investigate the applicability of Genotyping-by-Sequencing techniques in a sorghum mapping population generated between sweet stem and grain sorghum parents using two methods of Genotyping-by-Sequencing; whole genome shotgun (WGS) and restriction-site associated DNA (RAD). The results offered a robust framework for a precise and inexpensive Genotyping-by-Sequencing protocol through which sorghum populations can be characterised. 

I am currently busy with my PhD under the supervision of Prof Zander Myburg. My project is focused on investigating genome-wide characteristics of genetic architecture regulating growth and wood properties in a Eucalyptus grandis elite breeding population. Association studies have become one of the state-of-the-art biotechnological methods for detection of markers associated with trait variations. The main interest of this project is to find out which genetic variations and genome-wide haplotypes are associated with differences in cellulose, xylan and lignin biosynthesis as well as factors influencing fibre development in E. grandis. These results will contribute significantly to selection intensity for late expressing traits and characteristics that display low heritability in Eucalyptus species and E. grandis in particular.