Ms Julia Candotti
I am currently a PhD student in the Forest Molecular Genetics research programme. For my PhD, I aim to determine the haplotype and structural variant diversity present within Eucalyptus grandis and E. urophylla and how this diversity links to phenotype in F1 hybrids.
For my Masters research I generated genetic linkage maps and mapped QTLs in a Eucalyptus multi-parent mapping population. Multi-parent mapping populations combine the high power of linkage analysis with the high resolution of genome-wide association studies (GWAS) to identify quantitative trait loci (QTL). These populations are also advantageous as they allow for the analysis of segregation distortion of parental alleles across different full-sib and half-sib families which will allow for the identification of pre- and post-zygotic barriers to hybridisation. Recently, a Eucalyptus nested, multi-parent mapping population was created by crossing nine E. grandis pollen parents with eight E. urophylla seed parents. My study focused on one E. grandis half-sib and one E. urophylla half-sib. The aim of the study was to identify QTL underlying growth and wood properties through the use of genetic linkage maps and to identify regions of the parental genomes which preferentially combine. This study represented an important first step towards genetic dissection of hybrid combining ability and complex trait variation in E. grandis x E. urophylla multi-parent F1 hybrid trials.
As part of my MSc I presented my work at the following conferences:
Julia Candotti, Marja M. O’Neill, S. Melissa Reynolds, Roobavathie Naidoo, Nicoletta Jones, Eshchar Mizrachi, Alexander A. Myburg, Genetic dissection in a multi-parent Eucalyptus F1 hybrid mapping population, 23 - 28 June 2019, Raleigh,NC, USA (Poster, Lightening talk)
Candotti J, O’Neill MM, Reynolds SM, Naidoo R, Kanzler A, Jones N, Mizrachi E. Myburg AA. 2019. Genetic dissection of growth and wood development in nested, multi-parent Eucalyptus hybrid populations. Eucalypt genetics: fundamental and applied research in a post-genome era, 18-21 February 2019, Hobart, Tasmania, Australia (Speed Talk)
NATIONAL CONFERENCES (SOUTH AFRICA)
Candotti, J., O’Neill, M.M., Reynolds, S.M., Naidoo, S., Kanzler, A., Jones, N., Mizrachi, E., and Myburg, A.A. 2018. Towards nested, multi-parent genetic dissection of growth and wood development in Eucalyptus hybrid populations. SASBi/SAGs, 16-18 October 2018, Golden Gate resort, Free State, South Africa.(Poster)