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What to look for and collecting samples

Disease infection and spread

Ceratocystis species are fungal pathogens that affect mostly woody plant species.

Infection occurs through natural openings (buds, cracks in bark, root knots etc.) or external wounds on the stems or roots, induced through cultivation practices (e.g. grafting and pruning wounds), insects or weather-related conditions. The fungus invades the plant and blocks the water transport system resulting in wilting symptoms.

The spores of the fungus can be distributed via insects (e.g. picnic beetles or flies) by attaching to the insects which then transmit these spores from diseased to healthy plants. Some insect vectors bore into the diseased tissue of plants and the contaminated frass (borings) can be distributed via wind and rain.

Spread also occurs through the movement of infected plant material and soil. The thick fungal spore form can survive for long periods of time in the soil and infect new plants planted in the same soil.

Symptoms

Symptoms of infected plants include leaf discolouration (yellowing) and wilting (a) which can eventually lead to plant death (b). 

Internal infections in the cane/trunk are visible as dark brown streaks or mottled spots in the wood(c,d,e,f,h) or roots (g).

Ceratocystis wilt in Kiwi

Collecting samples for diagnosis

When wilting symptoms appear, inspect both rootstock, especially at the soil level, and recently wilted canes. Cutaway bark and look for streaking of wood (c,d,e,h).

Cut infected cane tissue at the margins of healthy and dying tissue or shave off thick pieces of discoloured wood with symptoms. Wrap samples from individual plants separately in newspaper and place them in separate plastic bags. Document variety number on bags. If roots are infected, first wash off soil and dry samples before wrapping in newspaper. 

Take clear photos of symptoms (use images here as examples).

If any symptoms are seen at the root collar, take soil samples. Mix up the soil (top 10cm) from around the base of the plant. Place ONE CUP of this soil in a plastic bag and seal firmly.

Send samples by COURIER to:

Dr Lieschen De Vos, (c/o Prof Irene Barnes/Prof Mike Wingfield)

Forestry and Agricultural Biotechnology Institute (FABI)

University of Pretoria

Lunnon Street Entrance

Hatfield

Pretoria

0083

Tel: 012 420 3938

Email: This email address is being protected from spambots. You need JavaScript enabled to view it. with courier tracking number, details and extent of the problem, photos, farm/collector details and contact numbers.

For more information on the FABI Diagnostic Clinic, please click here.

Download the pamphlet here

 

Text prepared by: Irene Barnes, Mike Wingfield

Photos by: Malcolm Deacon, Jolanda Roux and Irene Barnes

 

In the Applied Mycology Research Programme, we are focusing on to better understand the fungal communities that produce mycotoxins in agricultural crops, food and animal feed. Emphasis is placed on the diversity and taxonomy of genera such as Alternaria, Aspergillus, Fusarium, Penicillium and Talaromyces, but many others are also studied.  Ultimately, our goal is to secure food and feed from farm to fork for future generations.

The research group focusses on the interaction between cereal plants and foliar fungal pathogens. Pathosystems currently under study include grey leaf spot in maize (GLS) caused by Cercospora zeina, and northern corn leaf blight (NCLB) in maize and sorghum caused by Exserohilum turcicum. Projects are aimed at studying population structure of these pathogens, identification and functional characterisation of pathogen effectors causing disease, and cereal plants’ response to pathogen infection.

The Crop Floral Biology and Environments research team is focused on understanding how changing environments affect floral development, anthesis (flowering from bud opening to pollination) and pollination in economically important crops such as maize and sunflower. The multidisciplinary projects draw together aspects of climatology, plant physiology, plant molecular biology and ecology to holistically address these questions.

The CTHB promotes the health of trees indigenous to South Africa through the use of biotechnology. This is accomplished through projects that typically consider the pathogens and pests associated with native trees and woody hosts. The CTHB also explores the possible effects that factors such as climate change, society, natural forest health and plant genetics may have on the health of native woody resources and ecosystems.

 

The Grain Research Program (GRP) is a newly established programme that was developed by a team of multidisciplinary grain researchers from different institutes and programmes at FABI and elsewhere in South Africa. The GRP was formed to engage directly with grain stakeholders to initiate research that will support this sector by identifying future challenges to the industry and exploring solutions to these challenges. To its stakeholders in agriculture, the GRP also provides extension and disease/pest diagnostic services.

The Molecular Plant Physiology group collaborate with research groups in the UK, USA, Canada and Belgium to study the effect of biotic and abiotic stress in plants though a combination physiology, phenomics, functional genomics and proteomics. Using a holistic approach we aim to better understand the mechanisms underlying plant stress tolerance and resistance as well as the identification of potential biological markers that can then be used in plant improvement.

The Molecular Plant-Pathogen Interactions (MPPI) Group is located in the Plant Sciences Complex at the University of Pretoria and is headed by Prof Dave Berger. Research focuses on the molecular basis of plant-pathogen interactions using a range of approaches from whole plant phenotyping to functional genomics. We study the maize – grey leaf spot (Cercospora zeina) pathosystem.

The group is actively involved in seed science research. Our research fields include seed health tests, general seed pathology, seed vigour and seed germination according to ISTA rules. We are also involved in evaluating industry, or developing novel, environmentally sustainable, seed treatments. The research focuses on grains, herbs and vegetables.

The bacterial genomics and host pathogen interactions research group is interested in understanding molecular pathogen-host interactions between potato plants and their various pathogens. The objectives of the research include molecular identification of potato pathogens and pests, functional characterisation of bacterial virulence factors and elucidation of potato plant responses elicited by potato pathogens. We ultimately endevour to contribute knowledge toward the control of pathogens and pests under study.

The primary research focus of the Potato Pathology Programme is the epidemiology, diagnosis and control of soil- and seed-borne diseases of potatoes. Diseases currently being investigated include powdery scab, black scurf, stem canker, blackleg and soft rot. Various approaches are used to better understand disease spread and development, host-pathogen interactions and control, to provide growers with risk assessment and disease management strategies.  

 

 

Avocado Research Programme, a programme of the Forestry and Agricultural Biotechnology Institute (FABI), represents a cooperative venture between The Hans Merensky Foundation and the University of Pretoria, to deal with avocado disease problems, especially the avocado/Phytophthora cinnamomi interaction. HMF has committed a substantial amount to establish this research project at FABI and The University of Pretoria in turn provides the infrastructure necessary to conduct research.

 

The CTHB promotes the health of trees indigenous to South Africa through the use of biotechnology. This is accomplished through projects that typically consider the pathogens and pests associated with native trees and woody hosts. The CTHB also explores the possible effects that factors such as climate change, society, natural forest health and plant genetics may have on the health of native woody resources and ecosystems.

 

The Kiwifruit Protection Programme (KPP) was established in February 2023 as a collaborative initiative between the South African Kiwi Growers Association and the Forestry and Agricultural Biotechnology Institute (FABI). This programme, under the primary leadership of Prof. Irene Barnes, aims to support local kiwifruit farmers by conducting research on pests and diseases of kiwifruit and providing an extension and disease/pest diagnostic service to the farmers.

Macadamia is one of the fastest growing agricultural industries in the country and is of economic importance to South Africa. The industry is however hampered by a number of pests and diseases which can cause major economic loss, both in terms of yield as well as nut quality. The industry currently relies heavily on the use of commercially available pesticides and chemicals for pest and disease control. There is, however, growing concern regarding continuous build-up of resistance, and the long-term sustainability of the industry is dependent on the use of integrated pest management.

The Phytobacteriology programme at FABI has two main research areas, viz. taxonomy and pathogenicity. Several plant pathogenic bacteria are currently being characterized using a range of techniques, and are also being formally described. As one of our main focus areas, the pathogenicity factors of Panteoa ananatis, an opportunistic plant pathogen, are being investigated using a functional genomics approach.

New Publications

Botha I, Maduna SN, Hagen SB, Lall N, Berger DK. (2026) 3RAD-guided SNP discovery for species identification and conservation of the medicinal southern African tree Genus Greyia Hook. & Harv.. Ecology and Evolution 16(5):e73412, 1-29. 10.1002/ece3.73412
Jamieson B-A, Paap T, Pegg GS, Carnegie AJ, Wingfield MJ, Roux J, Hardy GEStJ, Drenth A, Hammerbacher A, Bose T. (2026) Quambalaria spp.: Emerging Tree Pathogens of Concern. Current Forestry Reports 12:13. 10.1007/s40725-026-00274-y
Masuku SK, De Vos L, Thabiso TE, Steenkamp ET, Wingfield BD. (2026) Baseline sensitivity of South African Fusarium circinatum to tebuconazole. Journal of Plant Pathology 10.1007/s42161-026-02197-w
Fitawek W, Anjulo A, Healey M, Lawson SA, Hurley BP. (2026) The Moringa value chain in Ethiopia and the socio-economic impact of pests and diseases. CABI Agriculture and Bioscience 10.1079/ab.2026.00041 PDF
Lynn KMT, Wingfield MJ, Oliveira LSS, Alfenas AC, Ferreira Alfenas RF, Marincowitz S, Barnes I. (2026) Phylogenetic and population genetic analyses reveal patterns of divergence amongst isolates of Ceratocystis manginecans. Ecology and Evolution 16 10.1002/ece3.73652
Dlamini CM, Matongera TN, Lawson SA, Healey M, Tanga A, Regasa K, Kassie W, Hurley BP, Germishuizen I. (2026) Modelling spatiotemporal dynamics of wattle plantations in northwestern Ethiopia using harmonised PlanetScope and RapidEye imagery. Trees, Forests and People 25:101293. 10.1016/j.tfp.2026.101293 PDF
Ndou M, Potts WM, Duong TA, Teske PR, Childs AR, Henriques R. (2026) Conspecific scaffold-level genome assembly outperforms heterospecific chromosome-level assemblies for assessing genetic indicators in a threatened marine fish. Evolutionary Applications 19:e70247. 10.1111/eva.70247
Marx B, van Dijk A, Steenkamp ET, Wingfield MJ, Wingfield BD. (2026) Breaking the mould: Cellulose in the cell walls of the Ophiostomatales. Fungal Biology Reviews 57 10.1016/j.fbr.2026.100489
Wingfield BD, Coetzee MPA, Wingfield BJ, Groenewald M, Pohl C, Wingfield MJ. (2026) The genetic blueprint of Cyclohexamide resistance: Analysis of 816 yeast species. Research Square 10.21203/rs.3.rs-9313202/v1
Paap T, White D, Bose T, Burgess TI. (2026) Diversity and phylogeny of Phytophthora Clade 9, including descriptions of three novel species. Mycological Progress 25:29. 10.1007/s11557-026-02140-4
Kgatla MM, Barker C, Baxter JR, Bester-van der Merwe AE, Chaisi M, Chakona A, Cherry MI, Daniels SR, Du Preez LH, Haddad CR, Hawkes PG, Ho C, Hoareau TB, Jacobs A, Jacobs K, Janion-Scheepers C, Jansen van Vuuren B, Kabongo RM, Khoza TT, Khumalo NL, Mahlanza T, Makapela L, Makhubo BG, Maneveldt GW, Mashego K, Matcher G, Matthee CA, Mavhunga M, Midgley JM, Mlambo M, Monsanto DM, Mthombeni R, Murray SL, Mynhardt S, Nang-Mba B, Ndlovu M, Parbhu SP, Phetla V, Phukuntsi M, Pitcher TR, Samaai T, Sethusa MT, Simon CA, Sink K, Sole CL, Theron GL, van Asch B, van der Bank M, van Steenderen CJM, Villet MH, Visagie CM, Williams KA, Willows-Munro S, Da Silva JM, Mwale M. (2026) An overview of DNA barcoding of biodiversity in South Africa. PLOS ONE 21(4):e0345173. 10.1371/journal.pone.0345173
Li GQ, Slippers B, Wingfield MJ, Chen SF. (2026) Diversity, distribution and host range of Botryosphaeriaceae in China. Forest Pathology 10.1111/efp.70077
Joubert M, van den Berg N, Theron J, Swart V. (2026) Leaf bleaching is associated with extensive transcriptional reprogramming in avocado trees with sunblotch disease. Virology 620:110903. 10.1016/j.virol.2026.110903 PDF
Nzuza P, Schroder ML, Slippers B, Maes WH. (2026) Spectral responses to larval and artificial defoliation in Eucalyptus dunnii: Implications for UAV-based detection of Gonipterus damage. Drones 10(4) 10.3390/drones10040250
Six DL, Marincowitz S, Duong TA. (2026) Ophiostoma ipsi-confusi sp. nov. Six, Marinc. & Duong, a consistent symbiotic fungus of the pinyon ips bark beetle, Ips confusus LeConte. Symbiosis 10.1007/s13199-026-01135-9
Yan Z, Zhao Y, Meng X, Gao M, Si H, Zhao G, Bose T, Chang R. (2026) New manganese-oxidizing Acremonium-like fungi from halophytic rhizospheres in the Yellow River Delta, China. Mycologia :1-13. 10.1080/00275514.2026.2627133
Eshetu FB, Barnes I, Nahrung HF, Fitza KNE, Slippers B. (2026) A Century of invasion: How biosecurity influenced populations of Sirex noctilio and Its fungal symbiont in Australasia. Molecular Ecology 35(6):0962 - 1083. 10.1111/mec.70311
Viljoen A, Duong TA, Kanzi AM, Wingfield BD. (2026) Comparative analysis of mitochondrial genomes in the Ceratocystidaceae reveals highly conserved gene organization despite substantial genome size variation. BMC Genomics 10.1186/s12864-026-12755-2
Ribeiro MF, Cavallini G, Solce GN, Favoreto AL, De Souza Passos J-R, Barbosa LR, Hurley BP, Wilcken CF. (2026) Cold storage of Gonipterus platensis (Coleoptera: Curculionidae) eggs for Anaphes nitens (Hymenoptera: Mymaridae) rearing. PeerJ :1-14. 10.7717/peerj.20903 PDF
Pham NQ, Marincowitz S, Marpaung YMAN, Tarigan M, Wingfield BD, Wingfield MJ. (2026) Two Cryphonectriaceae species from Eucalyptus leaves in North Sumatra and their stem inoculation outcomes. Fungal Systematics and Evolution 17:69–79. 10.15761/fuse.2026.17.05 PDF
Nickles GR, Stokes CK, Narh DL, Lynn KMT, Fuqua SR, Bryan C, Allen BM, Bivins CP, Bok JW, Brewer JS, Buthelezi ST, Clark JPRM, Coon KL, Corby LR, Coetzee MPA, Dewing C, Duong TA, Harris MA, Keller NP, Kopotsa K, Lane FA, Nichols HL, Nieuwoudt A, Nuñez MA, Medina Munoz ME, Park SC, Pham NQ, Ryan KT, Solís M, Vilgalys R, Wallace JM, Wang YW, Wingfield BD, Wingfield MJ, Worley TK, Zallek TA, Zamanian M, Hoeksma JD, Drott M, Pringle A. (2026) Equipped for success: Genomes and metabolites of the European Amanita muscaria are conserved in its novel South African range. New Phytologist 10.1111/nph.71064
Pham NQ, Wingfield MJ, Duong TA, Wingfield BD. (2026) Draft genome sequence of Elsinoe masingae: the causal agent of Eucalyptus scab in South Africa. Australasian Plant Pathology 55:37. 10.1007/s13313-026-01082-5
Pham NQ, Marincowitz S, Wingfield BD, Crous PW, Santos SA, Durán A, Tarigan M, Wingfield MJ . (2026) Pseudoteratosphaeria supramediana sp. nov. (Teratosphaeriaceae, Mycosphaerellales), a new foliar pathogen on Eucalyptus in Indonesia. Australasian Plant Pathology 55:28. 10.1007/s13313-026-01092-3
Nel WJ, Jali S, Barnes I, Wondafrash M, Hurley BP. (2026) Outbreaks of a native jewel beetle, Agrilus grandis (Coleoptera: Buprestidae), on commercial black wattle, Acacia mearnsii, plantations in South Africa. African Entomology 34(1):1-5. 10.17159/2254-8854/2026/a24625
Postma A, Klynsmith L, Duong TA, Allison JD, Smidt W, Waterhouse RM, Lesny P, Oeyen JP, Petersen M, Martin S, Liu S, Zhou X, Ziesmann T, Donath A, Mayer C, Misof B, Niehuis O, Peters RS, Podsiadlowski L, Coetzee MPA, Joubert F, Slippers B. (2026) Genome and transcriptome-based identification and expression profiling of chemosensory gene families across developmental stages and tissues in Sirex noctilio (Hymenoptera: Siricidae). Insect Molecular Biology :1-14. 10.1111/imb.70029
Aylward J, Visagie CM, Roets F, Wingfield BD, Wingfield MJ. (2026) Genome analyses reveal two novel species of Seiridium from Acacia mearnsii. Mycological Progress 25:8. 10.1007/s11557-026-02121-7
Liu QL, Wingfield MJ, Duong TA, Wingfield BD, Crous PW. (2026) Taxonomy, distribution and dispersal of Calonectria species: Important pathogens of forestry, agricultural and horticultural crops. Current Forestry Reports 12(4) 10.1007/s40725-025-00262-8
Nethononda PD, Hurley BP, Slippers B, Makhura MN. (2026) Smallholder farmers’ knowledge, perception and management of Spodoptera frugiperda (Lepidoptera: Noctuidae) on Zea mays at irrigation schemes in Limpopo province, South Africa. Crop Protection :107457. 10.1016/j.cropro.2025.107457
Swanepoel S, Naidoo S. (2026) A weighted gene co-expression network analysis characterises the common defence responses of Eucalyptus to diverse biotic challenges. Scientific Reports 16:5387. 10.1038/s41598-025-32699-z
Addikah C, Abubeker H, Mukiibi A, Bairu M, Amelework A, Van der Laan M, Mangani R. (2026) Current and future potential of cassava (Manihot esculenta) in Southern Africa: a scoping review. 4:120. 10.1007/s44279-026-00598-0
Botha I, De Canha MN, Oberlander K, Botes J, Lall N, Berger DK. (2025) DNA barcoding and anti-tyrosinase activities of three species-representative populations of the genus Greyia Hook & Harv. South African Journal of Botany 189:55-67. 10.1016/j.sajb.2025.11.035
Thomas C, Wilken PM, Coetzee MPA, Visagie CM. (2025) Advancing the taxonomy of Sclerotinia (Helotiales, Sclerotiniaceae): a review and recommendations for an important plant-pathogenic genus. IMA Fungus 17:e175737. 10.3897/imafungus.17.175737
Townsend G, Hill M, Hurley BP, Nel WJ, Crous C, Roets F. (2025) First report of the ambrosia beetle, Amasa parviseta (Curculiondae: Scolytinae), in South Africa. African Entomology 10.17159/2254-8854/2025/a24167 PDF
Roux J, del M Angel L, Barnes I. (2025) First report of the eucalypt stem canker pathogen Teratosphaeria gauchensis in South Africa. New Disease Reports 52(2):e70091. 10.1002/ndr2.70091
Nel WJ, Barnes I, Jali S, Impson F, Oberprieler RG, Hurley BP. (2025) First report of Melanterius inconspicuus (Coleoptera: Curculionidae: Cleogonini) from KwaZulu-Natal, South Africa, with observations of its development in black wattle. Southern Forests 10.2989/20702620.2025.2537823 PDF
Regasa K, Beze W, Anjulo A, Wondafrash M, Hurley BP, Lawson SA, Healey M, Germishuizen I. (2025) Evaluating fungicides for the management of rust (Uromycladium acaciae) on black wattle nursery seedlings in Awi zone, Amhara regional state, Ethiopia. International Journal of Forestry Research 10.1155/ijfr/5547814
Ramantswana TM, Malatji DP, Pierneef RE, Soma P, Van Der Nest MA, Muchadeyi FC. (2025) Differential gene expression analysis of Dohne Merino sheep naturally infected with Haemonchus contortus. Scientific Reports 15:41843. 10.1038/s41598-025-25782-y
Nadasen T, Buitendag C, Visser R, Welgemoed T, Hein I, Berger DK. (2025) A latent invader: transcriptomics reveals Cercospora zeina’s stealth infection strategy of maize and immune-activating effectors. Frontiers in Plant Science 16:1-23. 10.3389/fpls.2025.1703682
Wilson AM, Wingfield MJ, Duong TA, Wingfield BD. (2025) Thermotolerance and post-fire growth in Rhizina undulata is associated with the expansion of heat stress-related protein families. BMC Genomics 26:1041. 10.1186/s12864-025-11902-5
Fuchs T, Vismer HF, Visagie CM, Wingfield BD, Wingfield MJ. (2025) Low genotypic diversity and first reports of clinical Sporothrix from retrospective samples in South Africa. Medical Mycology 10.1093/mmy/myaf102
Yilmaz N, Verheecke-Vaessen C. (2025) Mycotoxins: An ongoing challenge to food safety and security. PLOS Pathogens 21(11) 10.1371/journal.ppat.1013672
Coelho MA, David-Palma M, Kachalkin AV, Kolařík M, Turchetti B, Sampaio JP, Wingfield MJ, Fisher MC, Yurkov AM, Heitman J. (2025) Genomic and phenotypic insights into the expanding phylogenetic landscape of the Cryptococcus genus. PLOS Genetics 21(11) 10.1371/journal.pgen.1011945 PDF
Ramatsitsi NM, Manyevere A, Motloba T. (2025) Myco-ecological warfare with Meloidogyne species. Archives of Agronomy and Soil Science 71(1):1-15. 10.1080/03650340.2025.2579892 PDF
Lane FA, Wingfield BD, Wingfield MJ, Wilken PM. (2025) Mating-type locus structure affects gene expression in unidirectional mating-type switching fungi. bioRxiv 10.1101/2025.10.10.681704
Shaw PL, Slippers B, Wingfield BD, Laurent B, Penaud B, Wingfield MJ, Crous PW, Bihon W, Duong TA. (2025) Chromosome-level genome assemblies for the latent pine pathogen, Diplodia sapinea, reveal two accessory chromosomes with distinct genomic features and evolutionary dynamics. G3 Genes|Genomes|Genetics :jkaf239. 10.1093/g3journal/jkaf239 PDF
Coelho MA, David-Palma M, Marincowitz S, Aylward J, Pham NQ, Yurkov AM, Wingfield BD, Wingfield MJ, Sheng S, Heitman J. (2025) The complex evolution and genomic dynamics of mating-type loci in Cryptococcus and Kwoniella. PLoS Biology 23:e3003417. 10.1371/journal.pbio.3003417
Visagie CM, Houbraken J, Overy DP, Sklenář F, Bensch K, Frisvad JC, Mack J, Perrone G, Samson RA, van Vuuren NI, Yilmaz N, Hubka V. (2025) From chaos to tranquillity: a modern approach to the identification, nomenclature and phylogeny of Aspergillus, Penicillium and other Eurotiales, including an updated accepted species list. Studies in Mycology 112:117–260. 10.3114/sim.2025.112.04
Vincent C, Singh A, Michalczyk GZ, Lane SL, Kaur R, Gill AR, Dziedzic N, De Silva K, Cho A, Cardoso AA, Alade DO, Tejera-Nieves M, Sharkey TD, Schmiege SC, Pelech E, Locke AM, Leisner CP, Teshome DT. (2025) Importance of measuring and reporting environmental conditions across plant science subdisciplines. Plant Physiology 199(2) 10.1093/plphys/kiaf405
Maake MM, Beukes CW, van der Nest MA, Avontuur JR, Muema EK, Stepkowski T, Venter SN, Steenkamp ET. (2025) Argyrolobium legumes from an African centre of endemism associate with novel Bradyrhizobium species harbouring unique sets of symbiosis genes. Molecular Phylogenetics and Evolution 214:108471. 10.1016/j.ympev.2025.108471
Joubert M, van den Berg N, Theron J, Swart V. (2025) Small RNAs derived from avocado sunblotch viroid and their association with bleaching symptoms: implications for pathogenesis in avocado sunblotch disease. Archives of Virology 170(10):205. 10.1007/s00705-025-06360-z PDF