Leptocybe invasa has a thelytokous reproductive system, where all offspring are female and are produced parthenogenetically. Male specimens are absent or very rare. Oviposition occurrs on young plant tissue of the stem, petiole and leaves as early as 1-2 weeks after bud break out. Eggs are laid in a line on the young plant tissue and are often visible as minute black scarring or dots, especially along the leaf midrib. Average developmental time from oviposition to wasp emergence is approximately 4.5 months.
Wasps are most active in the early morning and late afternoon and may survive to 6 days if provided with sources of honey and water. Oviposition occurs in the summer months with a single female being able to lay up 250 eggs.