The bacterial genomics and host pathogen interactions group is based at the University of Pretoria Department of Microbiology and Plant Pathology under the leadership of Dr Lucy Moleleki. Potato is an economically important crop plant contributing significantly to the South African economy. Pests and pathogens contribute significantly to annual production losses. Currently, the research group focuses on the Soft Rot Enterobacteriaceae and Root Knot Nematodes (Meloidogyne spp) of potatoes. To increase productivity, accurate identification of these pathogens is essential hence one of the focus areas of the research is molecular identification of potato pathogens. Pathogens overcome host defences by interfering with various host surveillance system. Hence, we endeavour to use genomics and transcriptomics to identify bacterial virulence factors as well as elucidate host defence mechanisms being targeted by these virulence factors.

Completed and Current project

Root Knot Nematodes

We recently completed a survery of root knot nematode species infecting potato plants in different growing regions across South Africa. In this survey the genetic diversity of the different Meloidogyne spp was profiled. Future studies will focus on understanding interactions of Meloidogyne spp and other potato pathogens as well as host plant potato.

Soft Rot Pathogens

We are currently using a variety of functional and comparative genomic tools to study the interaction of Pectobacterium spp in host plant potato during colonisation in a susceptible vs resistant host.

Group Leader: Dr Lucy Moleleki

New Publications

Chepsergon J, Moleleki LN. (2023) “Order from disordered”: Potential role of intrinsically disordered regions in phytopathogenic oomycete intracellular effector proteins. Current Opinion in Plant Biology 75:102402. 10.1016/j.pbi.2023.102402
Macharia TN, Duong TA, Moleleki LN. (2023) n silico secretome analyses of the polyphagous root-knot nematode Meloidogyne javanica: a resource for studying M. javanica secreted proteins. BMC Genomics 24:296. 10.1186/s12864-023-09366-6
Chepsergon J, Lucy N Moleleki. (2023) Rhizosphere bacterial interactions and impact on plant health. Current Opinion in Microbiology 73:102297. 10.1016/j.mib.2023.102297
Jane Chepsergon, Celiwe Nxumalo, Brenda Salasini, Aquillah Kanzi, Lucy Moleleki. (2022) Short Linear Motifs (SLiMs) in “Core” RxLR effectors of Phytophthora parasitica var. nicotianae: A case of PpRxLR1 effector. Microbiology Spectrum 10(2) 10.1128/spectrum.01774-21
Maphosa S and Moleleki LN. (2021) Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692. Microorganisms 9:1-20. 10.3390/ microorganisms9091918