High-throughput DNA Fingerprinting and Genotyping Research Service

The DNA Marker Ananlysis Facility is based in the Forest Molecular Genetics Laboratory at the University of Pretoria, and is supported by a team of technicians and geneticists.  The facility offers high-throughput plant DNA isolation, fingerprinting and genotyping research services focused on commercially grown tree species and occassionally other plant species on request.

 

The following research capacity is available:

  • DNA isolation from a range of plant tissues including leaves, pine needles, xylem scrapings, wood and bark cambium cores
  • Microsatellite DNA fingerprinting for most Eucalyptus and Pinus species grown in South Africa, using 10-20 microsatellite markers
  • Individual identification, clonal verification and parentage analysis for both Eucalyptus and pine species
  • Species discrimination for selected Eucalyptus and Pinus species

 

Contact information:

Ms Melissa Reynolds (This email address is being protected from spambots. You need JavaScript enabled to view it.) / 012-420 6874

Prof Zander Myburg (This email address is being protected from spambots. You need JavaScript enabled to view it.) / 012-420 4945

 

The QuantStudio 12K Flex Real-time PCR System

The QuantStudio™ 12K Flex system is a comprehensive real-time PCR-based platform for the fast, accurate and affordable investigation, validation and screening of hundreds of target genes and samples.  The QuantStudio™ system is highly flexible and includes five interchangeable blocks that can accommodate OpenArray plates, TaqMan array cards, 384-well plates which enable a researcher to scale up with the needs of individual experiments or projects.


The OpenArray system combined with the optimized pre-designed or custom TaqMan Assays is able to expand the number of samples and targets for maximum throughput analysis with its capacity to produce more than 12,000 high-quality, TaqMan data points per run.  The streamlined workflow and minimal hands-on time of OpenArray technology enables the efficient analysis of a number of applications including:

  • Large-scale gene expression analysis
  • SNP genotyping
  • microRNA analysis
  • digital PCR

 

The QuantStudio™ 12K Flex instrument can be found in room 6-28 in the Natural and Agricultural Sciences Building at the University of Pretoria.  Funding for the instrument was obtained from the NRF Equipment Platform (NEP) Grant. The instrument is thus a platform supporting genotyping and functional genomics projects on a national level.  THe use of hte instrument is available to researchers in South Africa.

 

For any queries or technical support please contact Stephan Henning (This email address is being protected from spambots. You need JavaScript enabled to view it.) or follow the link to the Life Technologies website.