The South African Soil SurveY (SASSY) aims to characterise fungal communities from soil using both culture-dependent and -independent methods. Collection sites are selected to best represent the nine biomes and 36 bioregions across the more than 100 nature reserves in South Africa. The first collections were made in the southern Cape during March. The second field trip was completed from 5-8 April by Prof. Cobus Visagie, Dr Neriman Yilmaz, Dr Anne Pringle and Mr David Johnson, and soil was collected from several reserves ranging from Hermanus to Wellington to Yzerfontein. We would like to thank the conservation managers for the assistance and advice they provided during our visit.