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Manager: DNA Sequencing Facility

Department

Genetics
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I am responsible for the DNA Sequencing Facility which provides a service to UP researchers. This facility houses ABI3130xl and ABI3500xl genetic analyzer instruments and also provides access to a Qubit fluorometer for quality control of samples (DNA, RNA, Protein).  We process Sanger sequencing samples and fragment analysis samples such as STR’s, tRFLP’s and SNP’s and offer technical and troubleshooting support for Sanger sequencing and Fragment analysis data.  The facility also hosts bi-annual Introductory Microsatellite workshop to assist our clients with all aspects of this technology in their laboratories.

I also co-manage the newly founded UP Ion Torrent Sequencing Facility together with Mr. Nicky Olivier. This facility aims to provide easy access to small scale next generation sequencing for UP researchers. This Ion Torrent PGM is particularly useful for bacterial and viral  genome sequencing as well as metagenomic analysis.

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Publication
Roets F, De Beer ZW, Dreyer LL, Zipfel R, Crous PW, Wingfield MJ. (2006) Multi-gene phylogeny for Ophiostoma spp. reveals two new species from Protea infructescences. Studies in Mycology 55:199-212. 10.3114/sim.55.1.199 PDF
Zipfel RD, De Beer ZW, Jacobs K, Wingfield BD, Wingfield MJ. (2006) Multi-gene phylogenies define Ceratocystiopsis and Grosmannia distinct from Ophiostoma. Studies in Mycology 55:75-97. 10.3114/sim.55.1.133 PDF